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See the Installation page in this manual for hints on acquiring, compiling and installing Phred & Phrap. In the context of Chromaseq, you will need to The programs Phred and Phrap are absolutely required for running For manual and verified tags, first line of comment field is displayed in output report.Before Phred and Phrap, scientists had to carefully look at discrepancies between overlapping DNA fragments; often, this involved manual determination of Phred. The phred software reads DNA sequencing trace files, calls bases, and assigns a quality value to each called base. Trimmomatic works with FASTQ files (using phred + 33 or phred + 64 quality scores, depending on the Illumina pipeline used). Files compressed using either Quality (Phred) scores · The quality score of a base, also known as a Phred or Q score, is an integer value representing the estimated probability of an error, CodonCode Aligner allows you to use the base calling program Phred and the assembly create "manual" folders to organize your samples and contigs.

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